A TRYPTOPHAN HISTOCHEMICAL METHOD

A revised method for histochemical demonstration of cytochrome oxidase.

A Histochemical Method for the Demonstration of Diphosphopyridine Nucleotide Diaphorase

The present investigation concerning the histochemical demonstration of DPN diaphorase follows the development of a new reagent, Nitro-BT, which has already been used successfully for the cytochemical localization of the succinic dehydrogenase system. The most consistently favorable results were obtained with the lactate-lactic dehydrogenase system buffered at pH 7.4. Using sections of rat kidn...

A fluorometric method for the estimation of tryptophan.

The various calorimetric methods now employed for the estimation of tryptophan are not specific for tryptophan but give colored products with many compounds containing the indole nucleus, including indole itself. In order to facilitate studies of the enzymatic synthesis of tryptophan from indole and serine by extracts of Neurospora (l), a search for a rapid, quantitative method for the estimati...

A chromatographic method for the detection of tryptophan metabolites.

During the course of investigations involving the action of tissue slices on tryptophan, the aqueous medium in which n-kynurenine sulfate had been incubated with kidney slices was found to have acquired a marked bluish violet fluorescence. Subsequent investigation showed that several known metabolites of tryptophan fluoresce when suitably exposed to ultraviolet light. Advantage has been taken o...

A Histochemical and Cytochemical Study

Localization of 5’-nucleotidase in the frog retina was investigated using histochemical and cytochemical techniques. Light-microscopic observations revealed the presence of this enzyme in the inner retinal layers (the nerve fiber layer, ganglion cell layer, and inner plexiform layer). Ultrastructural investigations revealed that the enzyme activity is associated with the plasma membranes of the...